Projects

(a) Regulation of hsp70 mRNA expression during chemical and “real” anoxia in the rainbow trout cell line RTHDF.

The project aims to investigate the enzymes responsible for the induction of the hsp70 gene and mRNA transcription after sublethal anoxia. We are comparing the effects of different methods for simulating anoxic conditions, by using sodium azide as a chemical model for anoxia and by O2-displacement using N2. Further, the effect of these treatments on intracellular signalling are studied.

(b) Regulation of the extracellular signal regulated protein kinase, ERK during anoxia and recovery.

The aim of this study is to determine signal transduction pathways involved in the previously described p38MAPK-dependent dephosphorylation of ERK during anoxia (Ossum et al. 2006).

(c) Proteom changes in RTHD-cells after anoxia and reoxygenation analysed by 2D-gel electrophoresis. Ph.d-student Tune Wulff is working on three articles based on data obtained during the project period: 1) Investigation of two different anoxia models by 2-Dimensional gel Electrophoresis, 2) Effects of long term anoxia on RTHDF cell-line investigated using an proteomic approach and 3) Genes changing in response to 12 and 24 hours of anoxia in the RTHDF cell-line.