Research school on Understanding bacterial interactions - and the potential use in sustainable control of fish diseases

Date: 18-24 April 2004

 

ECTS: 3 points

 

Venue
Danish Institute for Fisheries Research, Dept of Seafood Research

 

Course description

The course will through lecturers introduce the participants to disease problems in aquaculture with special emphasis on sustainable control. This will include the fish immune response, problems related to use of antibiotics as well as vaccination programmes. Major emphasis will be on bacterial interactions:

1) the selection and use of probiotic cultures and

2) the understanding of quorum sensing regulated bacterial communication and its potential use in disease control.

The objective is to give the Ph.D. students a basis for understanding interactions between bacteria relevant for fish diseases, and, to a limited extend, their interaction with the fish host. Practicals at the course will introduce the participants to AHL-signalling and to selection and testing of probiotic bacteria. All participants will be expected to prepared a short oral presentation of their own project.

 

Costs

There is no course fee, but you will have to make accommodation arrangements. A number of single and double rooms have been reserved at the Ph.D. hostel at the campus of the Danish Technical University. Hotel rooms are available in Lyngby (near-by town) or in Copenhagen (12 km - with good public transport), e.g. CabInn.

 

Further information

Professor Lone Gram or Secretary Christina Gundorff
Danish Institute for Fisheries Research,
Department of Seafood Research
Søltofts Plads
c/o Technical University of Denmark bldg. 221
DK-2800 Kgs. Lyngby
Denmark
phone: +45 45 25 25 86 or +45 45 88 33 22
fax: +45 45 88 47 74
e-mail or

 

You can also contact Professor Kurt Buchmann, Dept. of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1879 Frederiksberg C, Denmark; phone: +45 3528 2700; fax: +45 3528 2711; e-mail

 

Dead-line

Please download and complete this application form (MSWord .doc). Deadline for the formal application is April 1st 2004.

The course is intended for Ph.D. students, however, any vacancies are open for non-Ph.D. students. The individual lectures will be open to anyone interested.

The course is conducted under the auspice of the research school SCOFDA (Sustainable Control of Fish Diseases in Aquaculture).

Note that the course will be limited to 20 participants.

 

Program

Sunday 18th - Arrival
17-		Arrival, registration, get-together dinner + drinks
Monday 19th - Aquaculture and disease
9-10	LG	Welcome to course; short presentation of participants
10-11	KB	Aquaculture and disease problems (general)
11-12	BN	Fish diseases in Australian mariculture
12-13		Lunch
13-14	BN	Bacterial diseases of penaid prawns
14-15	LG, MRa, CBu, JBB	Introduction to practicals
15-17		Practicals Probiont Dilute, plate potential probionts (NB: inoculate Saturday) Sample, dilute and plate from fish skin, fish gills and fish intestine on non-selective plates (NB: also to be used for AHL-work) AHL Sub-culture fish pathogens Infect trout with V. anguillarum and other pathogens Inoculate AHL-monitor CV026 (Umeå strain)
Tuesday 20th - Diseases and treatments
9-10	ID	Important bacterial fish diseases
10-11	KB	Fish immune system - response to infections
11-12	BN	Role of bacteria in AGD in salmon
12-13		Lunch
13-14	MSB	Control of bacterial fish diseases; antibiotics and resistance problems
14-15	JLL	Control of bacterial fish disease; vaccines against bacteria
15-17		Practicals AHL Extract AHL compounds from fish pathogens Dissect diseased fish and extract AHL compounds Re-inoculate AHL-monitor in LB-medium with antibiotic (for TLC as well) Streak against AHL-monitor
Wednesday 21st - Presentations
9-10		Practicals TLC-profiling; start TLC-plates
10-12		Presentation and discussion of course participant projects
12-13		Lunch
13-14		Presentation and discussion of course participant projects
14-15
15-17		Practicals Probiont Cast plates with V. anguillarum as target organism Replica-plate from pure cultures of probionts and fish samples onto V. anguillarum plates AHL Cast AHL-monitor-plates and 1) test extracts (bacteria and fish) in well-diffusion 2) dry and agar-develop TLC-plates 3) replica-plate from natural fish surface samples
Thursday 22nd - Probiotics
9-10	LG	Antagonistic activity of microorganisms - use of biological control
10-11	MoJ	Human probiotics
11-12	TL	Probiotics in warm-blooded animals
12-13		Lunch
13-14	ON	Biocontrol in plant/rhizozphere communities
14-15	LG	Probiotics in fish and larval rearing
15-17		Practicals Probiont Streak inhibitory colonies on agar-plates and inoculate in broth Inoculate V. anguillarum for a well-diffusion assay
Friday 23rd - Quorum sensing
9-10	MG	Bacterial quorum sensing; regulation of virulence factors
10-11	JBB	AHLs in fish pathogens
11-12	DM	AHL-regulation of virulence factors in V. anguillarum
12-13		Lunch
13-14	TB	Quorum sensing inhibition - how to find and test
14-15	MRa	QS in fish pathogens - perspectives on QSI
15-17		Practicals Probiont Cast V. anguillarum well-assay plates Pipette isolated potential antagonists in plates Optional: Gram-reaction, motility, oxidase and catalase on streaked isolates. Inoculate in H&L-tubes AHL Read TLC-plates Read well-assay plates
Saturday 24th - Practicals, evaluation
10-11		Practicals Read TLC-plates Read well-diffusion agar plates Identify strains Discussion of results
11-12
12-13		Course evaluation
13-14		Lunch

Lecturers

Initials	Name	Title	Address
LG	Lone Gram	Professor	FF-DIFRES1
KB	Kurt Buchmann	Professor	LFD-KVL2
BN	Barbara Nowak	Senior Lecturer	University of Tasmania School of Aquaculture
MRa	Maria Rasch	Project researcher	FF-DIFRES1
CBu	Christiane Buch	Ph.D. student	FF-DIFRES1
ID	Inger Dalsgaard	Senior scientist	HØK-DIFRES1
MSB	Morten Sichlau Bruun	Scientist	HØK-DIFRES1/LFD-KVL2 /
JLL	Jens Laurits Larsen	Professor	LFD-KVL2
MoJ	Mogens Jakobsen	Professor	KVL2 Dept. of Dairy and Food Science
TL	Thomas D. Leser	Senior Scientist	Animal Health RD&A Chr. Hansen A/S
ON	Ole Nybroe	Associate Professor	G&M-KVL2
MG	Michael Givskov	Associate Professor	BioCentrum3
TB	Thomas Bovbjerg Rasmussen	Ph.D. student	BioCentrum3
DM	Debra Milton	Docent	Dept. of Molecular Biology Umeå University
JBB	Jesper B. Bruun

1. DIFRES: Danish Institute for Fisheries Research.
FF: Dept. of Seafood Research.
HØK: Dept. of Marine Ecology and Aquaculture.

2. KVL: The Royal Veterinary and Agricultural University.
G&M: Section of Genetics and Microbiology, Dept. of Ecology.
LFD: Lab. for Fish Diseases, Dept. of Veterinary Microbiology.

3. BioCentrum: Centre for Biomedical Microbiology, Technical Uni. of Denmark