Date: 18-24 April 2004
ECTS: 3 points
Venue
Danish Institute for Fisheries Research, Dept of Seafood Research
Course description
The course will through lecturers introduce the participants to disease problems in aquaculture with special emphasis on sustainable control. This will include the fish immune response, problems related to use of antibiotics as well as vaccination programmes. Major emphasis will be on bacterial interactions:
1) the selection and use of probiotic cultures and
2) the understanding of quorum sensing regulated bacterial communication and its potential use in disease control.
The objective is to give the Ph.D. students a basis for understanding interactions between bacteria relevant for fish diseases, and, to a limited extend, their interaction with the fish host. Practicals at the course will introduce the participants to AHL-signalling and to selection and testing of probiotic bacteria. All participants will be expected to prepared a short oral presentation of their own project.
Costs
There is no course fee, but you will have to make accommodation arrangements. A number of single and double rooms have been reserved at the Ph.D. hostel at the campus of the Danish Technical University. Hotel rooms are available in Lyngby (near-by town) or in Copenhagen (12 km - with good public transport), e.g. CabInn.
Further information
Professor Lone Gram or Secretary Christina Gundorff
Danish Institute for Fisheries Research,
Department of Seafood Research
Søltofts Plads
c/o Technical University of Denmark bldg. 221
DK-2800 Kgs. Lyngby
Denmark
phone: +45 45 25 25 86 or +45 45 88 33 22
fax: +45 45 88 47 74
e-mail or
You can also contact Professor Kurt Buchmann, Dept. of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1879 Frederiksberg C, Denmark; phone: +45 3528 2700; fax: +45 3528 2711; e-mail
Dead-line
Please download and complete this application form (MSWord .doc). Deadline for the formal application is April 1st 2004.
The course is intended for Ph.D. students, however, any vacancies are open for non-Ph.D. students. The individual lectures will be open to anyone interested.
The course is conducted under the auspice of the research school SCOFDA (Sustainable Control of Fish Diseases in Aquaculture).
Note that the course will be limited to 20 participants.
Program
Sunday 18th - Arrival
|
| 17- |
|
Arrival, registration, get-together dinner + drinks |
Monday 19th - Aquaculture and disease
|
| 9-10 |
LG |
Welcome to course; short presentation of participants |
| 10-11 |
KB |
Aquaculture and disease problems (general) |
| 11-12 |
BN |
Fish diseases in Australian mariculture |
| 12-13 |
|
Lunch |
| 13-14 |
BN |
Bacterial diseases of penaid prawns |
| 14-15 |
LG, MRa, CBu, JBB |
Introduction to practicals |
| 15-17 |
|
Practicals
Probiont
Dilute, plate potential probionts (NB: inoculate Saturday)
Sample, dilute and plate from fish skin, fish gills and fish intestine on non-selective plates (NB: also to be used for AHL-work)
AHL
Sub-culture fish pathogens
Infect trout with V. anguillarum and other pathogens
Inoculate AHL-monitor CV026 (Umeå strain) |
Tuesday 20th - Diseases and treatments
|
| 9-10 |
ID |
Important bacterial fish diseases |
| 10-11 |
KB |
Fish immune system - response to infections |
| 11-12 |
BN |
Role of bacteria in AGD in salmon |
| 12-13 |
|
Lunch |
| 13-14 |
MSB |
Control of bacterial fish diseases;
antibiotics and resistance problems |
| 14-15 |
JLL |
Control of bacterial fish disease;
vaccines against bacteria |
| 15-17 |
|
Practicals
AHL
Extract AHL compounds from fish pathogens
Dissect diseased fish and extract AHL compounds
Re-inoculate AHL-monitor in LB-medium with antibiotic (for TLC as well)
Streak against AHL-monitor |
Wednesday 21st - Presentations
|
| 9-10 |
|
Practicals
TLC-profiling; start TLC-plates |
| 10-12 |
|
Presentation and discussion of course participant projects |
| 12-13 |
|
Lunch |
| 13-14 |
|
Presentation and discussion of course participant projects |
| 14-15 |
|
| 15-17 |
|
Practicals
Probiont
Cast plates with V. anguillarum as target organism
Replica-plate from pure cultures of probionts and fish samples onto V. anguillarum plates
AHL
Cast AHL-monitor-plates and
1) test extracts (bacteria and fish) in well-diffusion
2) dry and agar-develop TLC-plates
3) replica-plate from natural fish surface samples |
Thursday 22nd - Probiotics
|
| 9-10 |
LG |
Antagonistic activity of microorganisms - use of biological control |
| 10-11 |
MoJ |
Human probiotics |
| 11-12 |
TL |
Probiotics in warm-blooded animals |
| 12-13 |
|
Lunch |
| 13-14 |
ON |
Biocontrol in plant/rhizozphere communities |
| 14-15 |
LG |
Probiotics in fish and larval rearing |
| 15-17 |
|
Practicals
Probiont
Streak inhibitory colonies on agar-plates and inoculate in broth
Inoculate V. anguillarum for a well-diffusion assay |
Friday 23rd - Quorum sensing
|
| 9-10 |
MG |
Bacterial quorum sensing; regulation of virulence factors |
| 10-11 |
JBB |
AHLs in fish pathogens |
| 11-12 |
DM |
AHL-regulation of virulence factors in V. anguillarum |
| 12-13 |
|
Lunch |
| 13-14 |
TB |
Quorum sensing inhibition - how to find and test |
| 14-15 |
MRa |
QS in fish pathogens - perspectives on QSI |
| 15-17 |
|
Practicals
Probiont
Cast V. anguillarum well-assay plates
Pipette isolated potential antagonists in plates
Optional:
Gram-reaction, motility, oxidase and catalase on streaked isolates. Inoculate in H&L-tubes
AHL
Read TLC-plates
Read well-assay plates |
Saturday 24th - Practicals, evaluation
|
| 10-11 |
|
Practicals
Read TLC-plates
Read well-diffusion agar plates
Identify strains
Discussion of results |
| 11-12 |
|
| 12-13 |
|
Course evaluation |
| 13-14 |
|
Lunch
|
Lecturers
| Initials |
Name |
Title |
Address |
| LG |
Lone Gram |
Professor |
FF-DIFRES1
|
| KB |
Kurt Buchmann |
Professor |
LFD-KVL2
|
| BN |
Barbara Nowak |
Senior Lecturer |
University of Tasmania
School of Aquaculture
|
| MRa |
Maria Rasch |
Project researcher |
FF-DIFRES1
|
| CBu |
Christiane Buch |
Ph.D. student |
FF-DIFRES1
|
| ID |
Inger Dalsgaard |
Senior scientist |
HØK-DIFRES1
|
| MSB |
Morten Sichlau Bruun |
Scientist |
HØK-DIFRES1/LFD-KVL2
/ |
| JLL |
Jens Laurits Larsen |
Professor |
LFD-KVL2
|
| MoJ |
Mogens Jakobsen |
Professor |
KVL2
Dept. of Dairy and Food Science
|
| TL |
Thomas D. Leser |
Senior Scientist |
Animal Health RD&A
Chr. Hansen A/S
|
| ON |
Ole Nybroe |
Associate Professor |
G&M-KVL2
|
| MG |
Michael Givskov |
Associate Professor |
BioCentrum3
|
| TB |
Thomas Bovbjerg Rasmussen |
Ph.D. student |
BioCentrum3
|
| DM |
Debra Milton |
Docent |
Dept. of Molecular Biology
Umeå University
|
| JBB |
Jesper B. Bruun |
|
|
1. DIFRES: Danish Institute for Fisheries Research.
FF: Dept. of Seafood Research.
HØK: Dept. of Marine Ecology and Aquaculture.
2. KVL: The Royal Veterinary and Agricultural University.
G&M: Section of Genetics and Microbiology, Dept. of Ecology.
LFD: Lab. for Fish Diseases, Dept. of Veterinary Microbiology.
3. BioCentrum: Centre for Biomedical Microbiology, Technical Uni. of Denmark